Research & Studies
Sports Performance Enhancement with Hydrogen Water
Deuterium Depleted Water
“The effect of deuterium depleted drinking water on the performance of sportsmen”
Hungarian Review of Sports Medicine (2005: 46/1:27-38.)
The authors saw an optimization in a host of parameters during the study period of 44 days. Twelve international-level male rowers participated in the study – 7 athletes consumed 2 L of 105 mg/L DDW water for 44 days and 5 athletes drank ordinary tap water. Both groups underwent a multistage load test at the start and end of the study. Pulse-rate was continuously monitored. Acid-base parameters (pH, pCO2, pO2, HCO3, base excess), ions (Na+, K+, Cl–), blood lactate and blood glucose, as well as the anion gap were determined from capillary blood samples.
The results of drinking deuterium-depleted water with 105 mg/L deuterium concentration:
Improved tissue oxygenation
Less pronounced tissue anoxia
Better mobilisation of glycose
Better utilisation of glucose; and
The cells showed a better metabolic compensation of the load-dependent changes in deuterium-depleted state achieved by consumption of 105 mg/L DDW.
Hydrogen-Rich Water
“Molecular hydrogen: a preventive and therapeutic medical gas for various diseases”
By Li Ge, Ming Yang et al. for:
Department of Histology and Embryology, School of Basic Medical Sciences, Taishan Medical University, Tai-an City, Shandong Province, China.
Department of Clinical Medicine, Taishan Medical University, Tai-an City, Shandong Province, China.
Key Laboratory of Atherosclerosis in Universities of Shandong, Taishan Medical University, Institute of Atherosclerosis, Taishan Medical University, Tai-an City, Shandong Province, China.
Department of medical immunology, School of Basic Medical Sciences, Taishan Medical University, Tai-an City, Shandong Province, China.nff
Hydrogen-Rich Water
“Molecular Hydrogen Affected Post-Exercise Recovery in Judo Athletes.”
Drid, Patrik et al.
Hydrogen-Rich Water
“Hydrogen-rich water affected blood alkalinity in physically active men”
Ostojic, S.M. and M.D. Stojanovic
Hydrogen-Rich Water
“Drinks with alkaline negative oxidative reduction potential (H2) improve exercise performance in physically active men and women: double-blind, randomised, placebo-controlled, cross-over trial of efficacy and safety”
Ostojić, S.M., et al.
Serbian journal of sports sciences, 2011. 5(1-4): p. 83-89.
Hydrogen-Rich Water
“Adequate hydration with hydrogen-rich water pre-exercise, reduced blood lactate levels and improved exercise-induced decline of muscle function.”
Aoki, K., et al.
Pilot study: “Effects of drinking hydrogen-rich water (is hydrogen water with an above average dissolved hydrogen content) on muscle fatigue caused by acute exercise in elite athletes.” Although acute exercise resulted in an increase in blood lactate levels in the subjects given regular water, oral intake of hydrogen rich water prevented an elevation of blood lactate during heavy exercise. Peak torque of subjects on regular water significantly decreased during maximal isokinetic knee extension, suggesting muscle fatigue, but peak torque of subjects on hydrogen rich water didn’t decrease at early phase.
Hydrogen-Rich Water
Molecular Hydrogen in Sports Medicine: New Therapeutic Perspectives
Int J Sports Med, 2014. (human) by: S.M Ostojic.
Review focusing on the applications of hydrogen in sports injury and sports performance enhancement.
Hydrogen-Rich Water
“Drinking hydrogen water enhances endurance and relieves psychometric fatigue: a randomized, double-blind, placebo-controlled study”.
Canadian Journal of Physiology and Pharmacology, 2019 Sep;97(9):857-862.
By: Toshio Mikami et al.
Endurance and fatigue were significantly improved in the H2 group as judged by maximal oxygen consumption and Borg’s scale, respectively. Taken together, drinking H2 water just before exercise exhibited anti-fatigue and endurance effects.
Anti-Aging, Pro-Youth and Skin Health
Hydrogen-Rich Water
“Molecular hydrogen: a preventive and therapeutic medical gas for various diseases”
By Li Ge, Ming Yang et al. for:
Department of Histology and Embryology, School of Basic Medical Sciences, Taishan Medical University, Tai-an City, Shandong Province, China.
Department of Clinical Medicine, Taishan Medical University, Tai-an City, Shandong Province, China.
Key Laboratory of Atherosclerosis in Universities of Shandong, Taishan Medical University, Institute of Atherosclerosis, Taishan Medical University, Tai-an City, Shandong Province, China.
Department of medical immunology, School of Basic Medical Sciences, Taishan Medical University, Tai-an City, Shandong Province, China.nff
Deuterium Depleted Water
“Anti-aging effects of deuterium depletion (with 90 mg/L DDW) on Mn-induced toxicity in a C. elegans model”
Daiana Silva Ávala, Gábor Somlyai et al.
This study confirms other references studies that shows, that a link between aging and Deuterium is well established in that D2O concentrations exceeding the natural level resulted in numerous adverse effects:
Reduced the life-span of mice (Czajka and Finkel, 1960)
Increased viral mutation rates (Konrad, 1960)
Deuteration of synthetic estrogen hormones weakened its estrogenic properties (Thompson, 1963)
Deuterated enzymes exhibited conformational changes, affecting their active sites (Van Hook, 1971)
The skin became enriched in deuterium along a temporal aging axis (Griffiths, 1973)
Deuterium Depleted Water
“Relation of antistress and geroprotective effects of deuterium depleted water in aging female rats.”
Oleg I. Kit , Alla I. Shikhliarova et al.
Drinking deuterium depleted water (DDW) for five weeks contributed to the overall correction of age-related disorders of the adaptive status, estrous cycle regulation and nonspecific resistance, as well as the appearance of visually distinct signs of rejuvenation of aging female rats (20–22 months).
Hydrogen-Rich Water
"Hydrogen enriched water improved mitochondria dysfunction."
Ito, M., et al.
Open-label trial and randomized, double-blind, placebo-controlled, crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies.
Hydrogen-Rich Water
“Hydrogen-rich Electrolyzed Warm Water Represses Wrinkle Formation Against UVA Ray Together With type-I Collagen Production and Oxidative-Stress Diminishment in Fibroblasts and Cell-Injury Prevention in Keratinocytes”
KATO, S., et al. (2012).
Hydrogen-Rich Water
"The Drinking Effect of Hydrogen Water on Atopic Dermatitis Induced by Dermatophagoides farinae Allergen in NC/Nga Mice."
Drinking hydrogen water suppressed the levels of inflammation-related mediators such as Th1, Th2, and pro-inflammatory cytokines which are number one players in the pathogenesis of human Atopic Dermatitis. In addition, this study provides a new insight of the relevance of certain cytokines in AD. Hydrogen Water represents a potentially alternative therapeutic and preventive treatment of Atopic Dermatitis.
Hydrogen-Rich Water
“Hydrogen(H2) treatment for acute erythematous skin diseases. A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers".
Ono, H., et al.
Erythema of these 4 patients and associated symptoms improved significantly after the Hydrogen treatment and did not recur. Administration of H2 did not change physiological parameters and did not cause deterioration of the blood chemistry.
Hydrogen-Rich Water
"Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro"
Li, Q., et al.
Twenty-two patients were retrospectively divided into an effective group (EG, n = 12) and a less effective group (LG, n = 10) according to the outcomes of endpoint evaluation and the healing criteria. PU hospitalized days in EG were significantly shorter than in LG (113.3 days vs. 155.4 days, p < 0.05), and the shortening rate was approximately 28.1%. Either in EG or in LG, the reducing changes (EG: 91.4%; LG: 48.6%) of wound size represented statistically significant difference versus before Hydrogen Water intake (p < 0.05, p < 0.001). The in vitro data demonstrate that intracellular ROS (Free radicals) as quantified by NBT assay was diminished by Hydrogen Water, but not by Regular Water.
Weight Loss, Metabolism & Inflammation
Deuterium Depleted Water
“Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats”
Tetiana Halenova et al.
This study shows a potential application of deuterium-depleted water (DDW)
for the prevention and adjuvant treatment of obesity in rats.
We tested the hypothesis that DDW can alleviate diet-induced obesity (DIO) and its associated metabolic impairments. Rats fed a high-fat diet had an increased body weight index (BWI), glucose concentration, and level of certain proinflammatory cytokines; decreased levels of insulin in the serum; decreased tryptophan and serotonin in the brain, and a decreased concentration of some heavy metals in the liver.
Drinking DDW at a concentration of 10 mg/L deuterium/protium (D/H) ad libitum for 3 weeks restored the BWI, glucose (serum), tryptophan (brain), and serotonin (brain) levels and concentration of Zn in the liver in the DIO animals to those of the controls.
The levels of proinflammatory cytokines (IL-1β, IL-6, IFNγ) and anti-inflammatory TNFα were decreased in DIO rats, while anti-inflammatory cytokine (IL-4, IL-10) levels remained at the control levels, which is indicative of a pathophysiological syndrome. In contrast, in groups of rats treated with DDW, a significant increase in anti-inflammatory (IL-4, IL-10) and proinflammatory cytokines (IFNγ) was observed.
This finding indicates a reduction in systemic inflammation in obese animals treated with DDW.
Similarly, the high-fat diet caused an increased level of oxidative stress products, which was accompanied by decreased activity of both superoxide dismutase and catalase, whereas the administration of DDW decreased the level of oxidative stress and enhanced antioxidant enzyme activities.
Hydrogen-Rich Water
"Molecular hydrogen improves obesity and diabetes…and stimulating energy metabolism in mice"
Naomi Kamimura et al.
Recent extensive studies have revealed that molecular hydrogen H2 has great potential for improving oxidative stress-related diseases by inhaling H2 gas, injecting saline with dissolved H2, or drinking hydrogen rich water; however, little is known about the dynamic movement of H2 in a body.
First, we show that liver glycogen accumulates H2 after oral administration of Hydrogen-rich water, explaining why consumption of even a small amount of hydrogen over a short span time efficiently improves various disease models.
The benefits of drinking hydrogen water ad libitum, on type 2 diabetes was investigated, using db/db obesity mice. Drinking Hydrogen water reduced oxidative stress in the liver, and significantly alleviated fatty liver in db/db mice as well as high fat-diet-induced fatty liver in wild-type mice.
Long-term drinking of hydrogen rich water significantly controlled fat and body weights…
Drinking Hydrogen water also decreased levels of plasma glucose, insulin, and triglyceride, the effect of which on hyperglycemia was similar to diet restriction!!!.
To examine how drinking Hydrogen rich water improves obesity and metabolic parameters at the molecular level, gene-expression profiles were examined.
The findings were that, indeed, hydrogen (H2) stimulated energy metabolism as measured by oxygen consumption. The present results suggest the potential benefit of Hydrogen (H2) in improving obesity, diabetes, and metabolic syndrome.
Cancer-related studies
Deuterium Depleted Water
“Submolecular regulation of cell transformation by deuterium depleting water exchange reactions in the tricarboxylic acid substrate cycle”
László Boros et al.
Deuterium depleted light water is able to protect DNA from radiation damage and the authors here establish a ling between mitochondrial deuterium load and dietary factors and cellular deuterium detoxification mechanisms…
“The naturally occurring isotope of hydrogen (1H), deuterium (2H), could have an important biological role. Deuterium depleted water delays tumor progression in mice, dogs, cats and humans. Hydratase enzymes of the tricarboxylic acid (TCA) cycle control cell growth and deplete deuterium from redox cofactors, fatty acids and DNA, which undergo hydride ion and hydrogen atom transfer reactions. A model is proposed that emphasizes the terminal complex of mitochondrial electron transport chain reducing molecular oxygen to deuterium depleted water (DDW); this affects gluconeogenesis as well as fatty acid oxidation. In the former, the DDW is thought to diminish the deuteration of sugar-phosphates in the DNA backbone, helping to preserve stability of hydrogen bond networks, possibly protecting against aneuploidy and resisting strand breaks, occurring upon exposure to radiation and certain anticancer chemotherapeutics. DDW is proposed here to link cancer prevention and treatment using natural ketogenic diets, low deuterium drinking water…”
Deuterium Depleted Water
“Deuterium Depleted Water Effects on Survival of Lung Cancer Patients and Expression of Kras, Bcl2, and Myc Genes in Mouse Lung”
Zoltán Gyöngyi
In a study of 129 lung cancer patients, who received deuterium depleted light water, together with regular treatment (chemo and radiation). On average, the survival was 200-400% greater than expected, especially in female patients, with survival of 74 months versus only 26 months.
Deuterium Depleted Water
“Deuterium Depletion May Delay the Progression of Prostate Cancer”
András Kovács and Imre Guller et al.
A clinical study looking at the effect of adding deuterium light water (DDW) along with conventional cancer treatment on 44 patients with prostate cancer.
Deuterium Depleted Water
“Deuterium Depleted Water Effects on Survival of Lung Cancer Patients and Expression of Kras, Bcl2, and Myc Genes in Mouse Lung”
Zoltán Gyöngyi et al.
Deuterium Depleted Water
“A retrospective study of survival in breast cancer patients undergoing deuterium depletion in addition to conventional therapies”
Krempels K. et al.
Study comparing the survival of breast cancer in groups of conventional therapy compared to a group drinking Deuterium Depleted Water in addition to conventional cancer therapy.
There is increasing evidence that the heavy isotope of hydrogen, deuterium (D), has a pivotal role in cell signalling and that deuterium depletion through the replacement of normal drinking water with deuterium-depleted water (DDW) results in tumour necrosis. The impact of Deuterium depletion on breast cancer outcome was studied retrospectively. The normal daily water intake (150 mg/L Deuterium) of 232 breast cancer patients was replaced with DDW (65-105 mg/L Deuterium) for at least 91 days, without altering conventional treatment regimens. According to staging at initial diagnosis, patients with early stage breast cancer achieved a median survival time (MST) of 18.1 years, compared with 4.3 years in patients with advanced disease. The MST is pending in the subgroup of patients who were in remission at the start of DDW treatmen
Deuterium Depleted Water
”Biological Significance of Naturally Occurring Deuterium: The Antitumor Effect of Deuterium Depletion”
Gábor Somlyai et al.
Deuterium depleted water (25 mg/L) induced complete or partial tumor regression in dogs and cats with spontaneous malignancies, it was registered as anticancer for veterinary use in 1999 (Vetera-DDW-25 A.U.V., 13/99 FVM). The hypodermic preparation of the registered veterinary drug was successfully tested in clinical investigations.
A randomized, double blind controlled, human Phase II clinical trial with prostate cancer (Under the Hungarian Institute of Pharmacology No. 5621/40/95) was performed, which exhibited a significant difference between the control and treated groups with respect to the examined parameters, median survival time and the extension of life-span.
The study suggests that cells are able to regulate the D/H ratio and the changes in the D/H ratio can trigger certain molecular mechanisms having a key role in cell cycle regulation. The study further supposes that not the shift in the intracellular pH, but the concomitant increase in the D/H ratio is the real trigger for the cells to enter into S phase. The decrease of Deuterium concentration can intervene in the signal transduction pathways thus leading to tumor regression.
Hydrogen-Rich Water
“Molecular hydrogen: a preventive and therapeutic medical gas for various diseases”
By Li Ge, Ming Yang et al. for:
* Department of Histology and Embryology, School of Basic Medical Sciences, Taishan Medical University, Tai-an City, Shandong Province, China.
* Department of Clinical Medicine, Taishan Medical University, Tai-an City, Shandong Province, China.
* Key Laboratory of Atherosclerosis in Universities of Shandong, Taishan Medical University, Institute of Atherosclerosis, Taishan Medical University, Tai-an City, Shandong Province, China.
* Department of medical immunology, School of Basic Medical Sciences, Taishan Medical University, Tai-an City, Shandong Province, China.nff
Hydrogen-Rich Water
"Molecular hydrogen and radiation protection."
Chuai, Y., et al. Free Radical Research, 2012. 46(9): p. 1061-7.
Molecular hydrogen (hydrogen water) acts as a therapeutic antioxidant by selectively reducing hydroxyl radicals (•OH) and peroxynitrite (ONOO-). It has been well-known that ionising radiation (IR) causes oxidative damage and consequent apoptosis (cell-death) mainly due to the production of •OH that follows radiolysis of H(2)O. Our department reported the protective effect of H(2) in irradiated cells and mice for the first time, and this effect is well repeated by us and another laboratory in different experimental animal models.
Hydrogen-Rich Water
"Hydrogen protects rats from dermatitis caused by local radiation."
Mei, K., et al.
Hydrogen significantly reduced the severity of dermatitis, accelerated tissue recovery, and reduced the extent of radiation-induced weight loss in rats after a single dose of 15 or 20 Gy but not 25 Gy of radiation. Hydrogen was also protective from cumulative doses of 30 Gy delivered in three fractions, respectively. Hydrogen also protect HaCaT cells from radiation-induced injury, it could significantly inhibit ionizing injury.
Hydrogen-Rich Water
“Neutral pH Hydrogen-Enriched Electrolyzed Water Achieves Tumor-Preferential Clonal Growth Inhibition Over Normal Cells and Tumor Invasion Inhibition Concurrently With Intracellular Oxidant Repression”.
Saitoh, Y., et al. Oncology Research, 2008. 17(6): p. 247-255.
Human tongue carcinoma cells were inhibited for either colony formation efficiencies or colony sizes by hydrogen water without significant inhibition to normal human tongue cells. Furthermore, hydrogen water caused growth inhibition, cell degeneration, and inhibition of invasion of human fibrosarcoma cells. Thus, neutral pH hydrogen water was shown to achieve tumor-preferential growth inhibition and tumor invasion together with scavenging of intracellular oxidants, and is expected as a preventive material against tumor progression and invasion.
Hydrogen-Rich Water
“Hydrogen-water enhances 5-fluorouracil-induced inhibition of colon cancer”
Runtuwene, J., et al. PeerJ, 2015. 3: p. e859.
“…Hydrogen water administration improved the survival of mice with colon 26-induced cancer. Furthermore, hydrogen water enhanced cell apoptosis in cancer cells, resulting in a marked increase in the expression of p-AMPK, AIF and Caspase 3 in colon 26 cells. Hydrogen water also increased the inhibitory effect of 5-FU on colon 26 cells with spect to cell survival rate and anticancer functions. Additionally, high-content hydrogen water exhibited stronger antioxidative and anticancer activity than did the natural hydrogen water.”
Hydrogen-Rich Water
"Response of five established solid transplantable mouse tumors and one mouse leukemia to hyperbaric hydrogen."
Roberts, B.J., et al. Cancer Treat Rep, 1978. 62(7): p. 1077-9.
Hydrogen-Rich Water
"Investigation of protective effect of hydrogen-rich water against chemotherapy-induced (cisplatin) nephrotoxicity in rats using blood oxygenation level-dependent magnetic resonance imaging."
Matsushita, T., et al. Jpn J Radiol, 2011. 29(7): p. 503-12.
Hydrogen-Rich Water
“Hyperbaric hydrogen therapy: a possible treatment for cancer”.
Dole, M., F.R. Wilson, and W.P. Fife. Science, 1975. 190(4210): p. 152-4.
“Mice with squamous cell carcinoma were exposed to a mixture of oxygen and hydrogen at a total pressure of 8 atmospheres for periods up to 2 weeks in order to see if a free radical decay catalyzer, such as hydrogen, would cause a regression of the skin tumors. Marked aggression of the tumors was found, leading to the possibility that hyperbaric hydrogen therapy might also prove to be of significance in the treatment of other types of cancer”.
Hydrogen-Rich Water
“Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors”.
Kang, K.-M., et al.
The consumption of hydrogen-rich water for 6 weeks reduced reactive oxygen metabolites in the blood and maintained blood oxidation potential. The Quality of Life (QOL) scores during radiotherapy were significantly improved in patients treated with hydrogen-rich water compared to patients receiving placebo water.
Hydrogen-Rich Water
“Hydrogen Gas in Cancer Treatment”.
Sai Li et al.
“…Growing evidence has shown that hydrogen gas can either alleviate the side effects caused by conventional chemotherapeutics, or suppress the growth of cancer cells and xenograft tumor, suggesting its broad potent application in clinical therapy. In the current review, we summarize these studies and discuss the underlying mechanisms.”
Protection against radiation, toxins and Free Radicals (ROS)
Deuterium Depleted Water
"Repair of damage caused by free radicals in the liver DDW combined with linseeds"
Repair of damage caused by free radicals in the liver
DDW combined with linseeds
Deuterium Depleted Water
"Protection liver against acetaminophen toxicity"
“In conclusion, our results revealed that DDW and DDW+ E.O have a potent hepatoprotective effect upon acetaminophen toxicity in rats by reducing the formation and detoxification of the active intermediate APAP metabolite”
Deuterium Depleted Water
"Cadmium pollution and associated cadmium toxicity. This study confirms the protective effect of Deuterium Depleted Water (DDW) in a rats."
Mihaela Doina Petcu et al.
“Variation of the Deuterium concentration in rats’ blood after deuterium depleted water administration and intoxication with Cadmium”
Deuterium Depleted Water
“Submolecular regulation of cell transformation by deuterium depleting water exchange reactions in the tricarboxylic acid substrate cycle”
László Boros et al.
Deuterium depleted light water is able to protect DNA from radiation damage and the authors here establish a ling between mitochondrial deuterium load and dietary factors and cellular deuterium detoxification mechanisms…
Deuterium Depleted Water
"The radioprotective effect of deuterium depleted water and polyphenols"
Gabriel C. Corneanu et al. in Environmental Engineering and Management Journal.
Study in mice confirmed the liver protective effect of deuterium depleted water in combination with polyphenol extract.
Deuterium Depleted Water
“Influence of Deuterium Depleted Water on the Organism of Laboratory Animals in Various Functional Conditions of Nonspecific Protective Systems”
A. B. Lisitsyn et al.
The impact of this water on biochemical indicators (AST, ALT, alkaline phosphatase, creatinine, bilirubin) and dynamics in body weight of laboratory animals within 42 days is established. As a result of studies the possibility of the preventive use of deuterium depleted water for correction of metabolic processes is shown in various conditions of the functional system of the body’s detoxification.
Deuterium Depleted Water
“Research Concerning the Radioprotective and Immunostimulating Effects of Deuterium-Depleted Water”
W. Bild et al.
Deuterium-depleted water was able to protect mice from the harmful effects of critical amounts of radioactive radiation and increased their chances of survival substantially, from only 25% without DDW, to 61% survival with DDW.
Hydrogen-Rich Water
"Molecular hydrogen and radiation protection."
Chuai, Y., et al. Free Radical Research, 2012. 46(9): p. 1061-7.
Molecular hydrogen (hydrogen water) acts as a therapeutic antioxidant by selectively reducing hydroxyl radicals (•OH) and peroxynitrite (ONOO-). It has been well-known that ionising radiation (IR) causes oxidative damage and consequent apoptosis (cell-death) mainly due to the production of •OH that follows radiolysis of H(2)O. Our department reported the protective effect of H(2) in irradiated cells and mice for the first time, and this effect is well repeated by us and another laboratory in different experimental animal models.
Comment: This may show to be a relevant study in other radiation models, such as in strong radiation fields generated by microwave or mobile radiation in the 5 GHz range, as currently debated internationally.
Hydrogen-Rich Water
"Neuroprotective effect of hydrogen-rich saline in acute carbon monoxide poisoning."
Shen, M.H., et al.
“…hydrogen rich saline may be clinically useful against acute CO poisoning, possibly via reducing (Free Radicals) ROS level, up-regulating endogenous antioxidative enzymes, suppressed caspase enzyme activity, upregulated ratio of Bcl‐2 and Bax expression, and abated apoptosis.
Hydrogen-Rich Water
"Long-term treatment of hydrogen-rich saline abates testicular oxidative stress induced by nicotine in mice."
Li, S., et al.
It was found that vitamin C and vitamin E attenuated serum oxidative level, but did not lower testicular oxidative levels in mice subjected to chronic nicotine treatment, and did not improve the male reproductive damage and apoptosis induced by nicotine. Different from normal antioxidants, vitamin C and vitamin E, hydrogen-rich saline (hydrogen rich water) abated oxidative stress in testis, and protected against nicotine-induced male reproductive damages.
Hydrogen-Rich Water
"Hydrogen protects rats from dermatitis caused by local radiation."
Mei, K., et al.
Hydrogen significantly reduced the severity of dermatitis, accelerated tissue recovery, and reduced the extent of radiation-induced weight loss in rats after a single dose of 15 or 20 Gy but not 25 Gy of radiation. Hydrogen was also protective from cumulative doses of 30 Gy delivered in three fractions, respectively. Hydrogen also protect HaCaT cells from radiation-induced injury, it could significantly inhibit ionizing injury.
Brain Function Study
Deuterium Depleted Water
“Deuterium-depleted water has stimulating effects on long-term memory in rats”
Cristian Mladin et al.
Deuterium-depleted water (DDW) is a water which has a 6-7-fold less concentration of the naturally occurring deuterium (20-25mg/L vs. 150mg/L). While administrated for a longer period, it may reduce the concentration of deuterium throughout the body, thus activating cellular mechanisms which are depending on protons (channels, pumps, enzyme proteins). The aim of the present work was to study, for the first time in our knowledge, the possible influence of deuterium-depleted water (DDW) chronic administration in normal Wistar rats, as compared to a control group which received distilled water, on spatial working memory and the locomotor activity (as studied through Y-maze) or both short-term and long-term spatial memory (assed in radial 8 arms-maze task).
…we could speculate that the administration of DDW may generate an improvement of the reference memory, as an index of long-term memory. Thus, we can reach the conclusion that the change between the deuterium/hydrogen balance may have important consequences for the mechanisms that govern long-term memory, as showed here especially in the behavioral parameters from the eight-arm radial maze task.
Deuterium Depleted Water
“Deuterium content of water increases depression susceptibility: the potential role of a serotonin-related mechanism”
Tatyana Strekalova et al.
The study investigated the possible link between deuterium content of water and depression, both epidemiologically, and in a mouse model of chronic mild stress.
The study showed that deuterium depletion through the use of deuterium depleted water, decreased depression score, gave more restful sleep and sharpened wakefulness.
Hydrogen-Rich Water
“Molecular hydrogen: a preventive and therapeutic medical gas for various diseases”
By Li Ge, Ming Yang et al. for:
Department of Histology and Embryology, School of Basic Medical Sciences, Taishan Medical University, Tai-an City, Shandong Province, China.
Department of Clinical Medicine, Taishan Medical University, Tai-an City, Shandong Province, China.
Key Laboratory of Atherosclerosis in Universities of Shandong, Taishan Medical University, Institute of Atherosclerosis, Taishan Medical University, Tai-an City, Shandong Province, China.
Department of medical immunology, School of Basic Medical Sciences, Taishan Medical University, Tai-an City, Shandong Province, China.nff
Hydrogen-Rich Water
"Hydrogen-rich saline reverses oxidative stress, cognitive impairment, and mortality in rats submitted to sepsis by cecal ligation and puncture."
Zhou, J., et al.,
Hydrogen-Rich Water
“Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer’s disease by reduction of oxidative stress.”
Li, J., et al.
“In conclusion, hydrogen-rich saline prevented Abeta-induced neuroinflammation and oxidative stress, which may contribute to the improvement of memory dysfunction in this rat model”.
Hydrogen-Rich Water
"Drinking hydrogen water and intermittent hydrogen gas exposure, but not lactulose or continuous hydrogen gas exposure, prevent 6-hydorxydopamine-induced Parkinson’s disease in rats."
Ito, M., et al.
Hydrogen water; and intermittent exposure to 2% hydrogen gas prevented the development of 6-OHDA induced Parkinson’s Disease in a rats.
Hydrogen-Rich Water
“Oral ‘hydrogen water’ induces neuroprotective ghrelin secretion in mice“.
Akio Matsumoto et al.
The study demonstrated that supplementation with hydrogen-rich water resulted in an increased production of the hormone ghrelin (which is a hormone of interest in relation to sleep, appetite, memory, depression and even cancer due to its anti-inflammatory and anti-apoptotic potential).
Hydrogen-Rich Water
“Hydrogen is neuroprotective against surgically induced brain injury“.
Jan M. Eckermann et al. Medical Gas Research, 2011. 1(1): p. 7.
Neurosurgical operations cause unavoidable damage to healthy brain tissues. Direct surgical injury as well as surgically induced oxidative stress contributes to the subsequent formation of brain edema. Therefore, we tested the neuroprotective effects of hydrogen (H2) in rats.
Hydrogen (2.9%) administered concurrently with surgery significantly decreased the formation of cerebral edema (p = 0.028) and improved neurobehavioral score (p = 0.022)…Hydrogen appears to be promising as an effective, yet inexpensive way to reduce cerebral edema caused by surgical procedures. Hydrogen has the potential to improve clinical outcome, decrease hospital stay, and reduce overall cost to patients and the health care system.
Hydrogen-Rich Water
“Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson’s disease“.
Yuan Fu et al.
Hydrogen-rich water is a selective antioxidant that reduces hydroxyl radicals, but not the other reactive oxygen and nitrogen species… In Parkinson’s disease, mitochondrial dysfunction and the associated oxidative stress, are major causes of dopaminergic cell loss… “Our studies suggest that hydrogen water is likely able to retard the development and progression of Parkinson’s disease”.